Monday, 22 September 2025 4:00pm – 5:00pm

This seminar will be delivered in Lecture Theatre 4

Speaker: A/Prof. Magnus Andersson

Host: Prof. Ron Clarke

Title: Tracking P-type ATPase native structural dynamics in real time

Abstract
P-type ATPase transport is carried out in a complex lipid environment and is regulated by both external and internal factors. We have developed a methodology to track P-type ATPase domain rearrangements in real time triggered by laser-induced release of ATP and subsequent monitoring by synchrotron X-ray pulses. We used this time-resolved X-ray solution scattering (TR-XSS) technique to capture the dynamics of eukaryotic and prokaryotic calcium transport (SERCA and LMCA1). We explored regulatory dynamics induced by the transported calcium ions, and also the surrounding lipid/detergent environment. We developed structural refinement approached based on MD simulations and ensemble learning algorithms. The results show both subtle and drastic effects on the protein dynamics and kinetics induced by calcium and lipid/detergent regulatory processes.

Bio

• Associate Professor, Department of Chemistry, Umeå University (appointed in 2018)
• Aug 2013-March 2018 Researcher, Science for Life Laboratory, Dept. of Theoretical Physics,
• KTH Royal Institute of Technology, Sweden
• Aug 2012-Sep 2013 Researcher, Dept. of Biochemistry and Biophysics, Stockholm University, Sweden
• Aug 2009-July 2012 Postdoctoral researcher, Dept. of Physiology and Biophysics                         University of California, Irvine, USA
• Jan 2009-July 2009 Postdoctoral researcher, Centre of Molecular Movies (CMM), Niels Bohr Institute, University of Copenhagen, Denmark

Educational qualifications

• Dec 2008     D. Bioscience, Dept. of Chemistry and Bioscience, Chalmers University of Technology, Gothenburg, Sweden. Thesis title: Visualizing structural dynamics in solution: from small molecules to membrane proteins. Main supervisor: Richard Neutze
• Apr 2003     Sc. Chemistry, Gothenburg University, Gothenburg, Sweden
  External diploma work at University of New South Wales, Sydney, Australia